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dna restriction fragment length polymorphism rflp  (New England Biolabs)
96
New England Biolabs dna restriction fragment length polymorphism rflp
Dna Restriction Fragment Length Polymorphism Rflp, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glutathione sepharose 4b  (GE Healthcare)
99
GE Healthcare glutathione sepharose 4b
Glutathione Sepharose 4b, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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clobetasol propionate emollient 0.05  (Becton Dickinson)
90
Becton Dickinson clobetasol propionate emollient 0.05
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anti stat5b c 17  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology anti stat5b c 17
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jnk1  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology jnk1
(A) The knockout of TRADD in DG75 B lymphocytes abolishes TNFR1 activation of the NF-κB pathway. DG75 TRADD +/+ or TRADD –/– cells were stimulated with 200 ng ml −1 soluble human TNFα (Roche) for the indicated times. Levels of phospho-I-κB and I-κB were analyzed on immunoblots. αTubulin served as the loading control. (B) LMP1 activation of IKKβ requires TRADD. DG75 TRADD +/+ or TRADD –/– cells were electroporated with the indicated LMP1 constructs and Flag-IKKβ. Flag-IKKβ activity was monitored in immunocomplex kinase assays. GST-I-κBα phosphorylation was quantified by a phosphoimager and is given as x-fold induction. Immunoprecipitated Flag-IKKβ and the expressed HA-LMP1 constructs were detected using the anti-Flag (M2) and anti-HA (12CA5) antibodies, respectively. LMP1-CD40 was visualized by the anti-CD40 antibody. IB, immunoblot; IP, immunoprecipitation. (C) Ectopic TRADD expression rescues LMP1 activation of IKKβ in TRADD–/– cells. IKKβ assays were performed in DG75 TRADD–/– cells as in (B). Where indicated, TRADD was expressed at physiological levels by co-transfecting 1 μg of pACYC184-1012.4, which carries the complete human TRADD gene including the TRADD promoter. (D) <t>JNK1</t> activation by LMP1 is independent of TRADD. HA-JNK1 immunocomplex kinase assays in DG75 TRADD +/+ and TRADD –/– cells are shown. Immunoprecipitated HA-JNK1 was dectected with the anti-JNK1 (C17) antibody.
Jnk1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibodies monoclonal anti stat5 w 17  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology antibodies monoclonal anti stat5 w 17
(A) The knockout of TRADD in DG75 B lymphocytes abolishes TNFR1 activation of the NF-κB pathway. DG75 TRADD +/+ or TRADD –/– cells were stimulated with 200 ng ml −1 soluble human TNFα (Roche) for the indicated times. Levels of phospho-I-κB and I-κB were analyzed on immunoblots. αTubulin served as the loading control. (B) LMP1 activation of IKKβ requires TRADD. DG75 TRADD +/+ or TRADD –/– cells were electroporated with the indicated LMP1 constructs and Flag-IKKβ. Flag-IKKβ activity was monitored in immunocomplex kinase assays. GST-I-κBα phosphorylation was quantified by a phosphoimager and is given as x-fold induction. Immunoprecipitated Flag-IKKβ and the expressed HA-LMP1 constructs were detected using the anti-Flag (M2) and anti-HA (12CA5) antibodies, respectively. LMP1-CD40 was visualized by the anti-CD40 antibody. IB, immunoblot; IP, immunoprecipitation. (C) Ectopic TRADD expression rescues LMP1 activation of IKKβ in TRADD–/– cells. IKKβ assays were performed in DG75 TRADD–/– cells as in (B). Where indicated, TRADD was expressed at physiological levels by co-transfecting 1 μg of pACYC184-1012.4, which carries the complete human TRADD gene including the TRADD promoter. (D) <t>JNK1</t> activation by LMP1 is independent of TRADD. HA-JNK1 immunocomplex kinase assays in DG75 TRADD +/+ and TRADD –/– cells are shown. Immunoprecipitated HA-JNK1 was dectected with the anti-JNK1 (C17) antibody.
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rabbit polyclonal ac k2 hmgn2 antibody  (Biosynth Carbosynth)
86
Biosynth Carbosynth rabbit polyclonal ac k2 hmgn2 antibody
(A) The knockout of TRADD in DG75 B lymphocytes abolishes TNFR1 activation of the NF-κB pathway. DG75 TRADD +/+ or TRADD –/– cells were stimulated with 200 ng ml −1 soluble human TNFα (Roche) for the indicated times. Levels of phospho-I-κB and I-κB were analyzed on immunoblots. αTubulin served as the loading control. (B) LMP1 activation of IKKβ requires TRADD. DG75 TRADD +/+ or TRADD –/– cells were electroporated with the indicated LMP1 constructs and Flag-IKKβ. Flag-IKKβ activity was monitored in immunocomplex kinase assays. GST-I-κBα phosphorylation was quantified by a phosphoimager and is given as x-fold induction. Immunoprecipitated Flag-IKKβ and the expressed HA-LMP1 constructs were detected using the anti-Flag (M2) and anti-HA (12CA5) antibodies, respectively. LMP1-CD40 was visualized by the anti-CD40 antibody. IB, immunoblot; IP, immunoprecipitation. (C) Ectopic TRADD expression rescues LMP1 activation of IKKβ in TRADD–/– cells. IKKβ assays were performed in DG75 TRADD–/– cells as in (B). Where indicated, TRADD was expressed at physiological levels by co-transfecting 1 μg of pACYC184-1012.4, which carries the complete human TRADD gene including the TRADD promoter. (D) <t>JNK1</t> activation by LMP1 is independent of TRADD. HA-JNK1 immunocomplex kinase assays in DG75 TRADD +/+ and TRADD –/– cells are shown. Immunoprecipitated HA-JNK1 was dectected with the anti-JNK1 (C17) antibody.
Rabbit Polyclonal Ac K2 Hmgn2 Antibody, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fidelity pcr master mix  (New England Biolabs)
99
New England Biolabs fidelity pcr master mix
(A) The knockout of TRADD in DG75 B lymphocytes abolishes TNFR1 activation of the NF-κB pathway. DG75 TRADD +/+ or TRADD –/– cells were stimulated with 200 ng ml −1 soluble human TNFα (Roche) for the indicated times. Levels of phospho-I-κB and I-κB were analyzed on immunoblots. αTubulin served as the loading control. (B) LMP1 activation of IKKβ requires TRADD. DG75 TRADD +/+ or TRADD –/– cells were electroporated with the indicated LMP1 constructs and Flag-IKKβ. Flag-IKKβ activity was monitored in immunocomplex kinase assays. GST-I-κBα phosphorylation was quantified by a phosphoimager and is given as x-fold induction. Immunoprecipitated Flag-IKKβ and the expressed HA-LMP1 constructs were detected using the anti-Flag (M2) and anti-HA (12CA5) antibodies, respectively. LMP1-CD40 was visualized by the anti-CD40 antibody. IB, immunoblot; IP, immunoprecipitation. (C) Ectopic TRADD expression rescues LMP1 activation of IKKβ in TRADD–/– cells. IKKβ assays were performed in DG75 TRADD–/– cells as in (B). Where indicated, TRADD was expressed at physiological levels by co-transfecting 1 μg of pACYC184-1012.4, which carries the complete human TRADD gene including the TRADD promoter. (D) <t>JNK1</t> activation by LMP1 is independent of TRADD. HA-JNK1 immunocomplex kinase assays in DG75 TRADD +/+ and TRADD –/– cells are shown. Immunoprecipitated HA-JNK1 was dectected with the anti-JNK1 (C17) antibody.
Fidelity Pcr Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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q5 high fidelity polymerase  (New England Biolabs)
99
New England Biolabs q5 high fidelity polymerase
(A) The knockout of TRADD in DG75 B lymphocytes abolishes TNFR1 activation of the NF-κB pathway. DG75 TRADD +/+ or TRADD –/– cells were stimulated with 200 ng ml −1 soluble human TNFα (Roche) for the indicated times. Levels of phospho-I-κB and I-κB were analyzed on immunoblots. αTubulin served as the loading control. (B) LMP1 activation of IKKβ requires TRADD. DG75 TRADD +/+ or TRADD –/– cells were electroporated with the indicated LMP1 constructs and Flag-IKKβ. Flag-IKKβ activity was monitored in immunocomplex kinase assays. GST-I-κBα phosphorylation was quantified by a phosphoimager and is given as x-fold induction. Immunoprecipitated Flag-IKKβ and the expressed HA-LMP1 constructs were detected using the anti-Flag (M2) and anti-HA (12CA5) antibodies, respectively. LMP1-CD40 was visualized by the anti-CD40 antibody. IB, immunoblot; IP, immunoprecipitation. (C) Ectopic TRADD expression rescues LMP1 activation of IKKβ in TRADD–/– cells. IKKβ assays were performed in DG75 TRADD–/– cells as in (B). Where indicated, TRADD was expressed at physiological levels by co-transfecting 1 μg of pACYC184-1012.4, which carries the complete human TRADD gene including the TRADD promoter. (D) <t>JNK1</t> activation by LMP1 is independent of TRADD. HA-JNK1 immunocomplex kinase assays in DG75 TRADD +/+ and TRADD –/– cells are shown. Immunoprecipitated HA-JNK1 was dectected with the anti-JNK1 (C17) antibody.
Q5 High Fidelity Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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acetonitrile acn  (Biosynth Carbosynth)
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Biosynth Carbosynth acetonitrile acn
(A) The knockout of TRADD in DG75 B lymphocytes abolishes TNFR1 activation of the NF-κB pathway. DG75 TRADD +/+ or TRADD –/– cells were stimulated with 200 ng ml −1 soluble human TNFα (Roche) for the indicated times. Levels of phospho-I-κB and I-κB were analyzed on immunoblots. αTubulin served as the loading control. (B) LMP1 activation of IKKβ requires TRADD. DG75 TRADD +/+ or TRADD –/– cells were electroporated with the indicated LMP1 constructs and Flag-IKKβ. Flag-IKKβ activity was monitored in immunocomplex kinase assays. GST-I-κBα phosphorylation was quantified by a phosphoimager and is given as x-fold induction. Immunoprecipitated Flag-IKKβ and the expressed HA-LMP1 constructs were detected using the anti-Flag (M2) and anti-HA (12CA5) antibodies, respectively. LMP1-CD40 was visualized by the anti-CD40 antibody. IB, immunoblot; IP, immunoprecipitation. (C) Ectopic TRADD expression rescues LMP1 activation of IKKβ in TRADD–/– cells. IKKβ assays were performed in DG75 TRADD–/– cells as in (B). Where indicated, TRADD was expressed at physiological levels by co-transfecting 1 μg of pACYC184-1012.4, which carries the complete human TRADD gene including the TRADD promoter. (D) <t>JNK1</t> activation by LMP1 is independent of TRADD. HA-JNK1 immunocomplex kinase assays in DG75 TRADD +/+ and TRADD –/– cells are shown. Immunoprecipitated HA-JNK1 was dectected with the anti-JNK1 (C17) antibody.
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dhea-s enzyme immunoassay kit  (Salimetrics LLC)
90
Salimetrics LLC dhea-s enzyme immunoassay kit
(A) The knockout of TRADD in DG75 B lymphocytes abolishes TNFR1 activation of the NF-κB pathway. DG75 TRADD +/+ or TRADD –/– cells were stimulated with 200 ng ml −1 soluble human TNFα (Roche) for the indicated times. Levels of phospho-I-κB and I-κB were analyzed on immunoblots. αTubulin served as the loading control. (B) LMP1 activation of IKKβ requires TRADD. DG75 TRADD +/+ or TRADD –/– cells were electroporated with the indicated LMP1 constructs and Flag-IKKβ. Flag-IKKβ activity was monitored in immunocomplex kinase assays. GST-I-κBα phosphorylation was quantified by a phosphoimager and is given as x-fold induction. Immunoprecipitated Flag-IKKβ and the expressed HA-LMP1 constructs were detected using the anti-Flag (M2) and anti-HA (12CA5) antibodies, respectively. LMP1-CD40 was visualized by the anti-CD40 antibody. IB, immunoblot; IP, immunoprecipitation. (C) Ectopic TRADD expression rescues LMP1 activation of IKKβ in TRADD–/– cells. IKKβ assays were performed in DG75 TRADD–/– cells as in (B). Where indicated, TRADD was expressed at physiological levels by co-transfecting 1 μg of pACYC184-1012.4, which carries the complete human TRADD gene including the TRADD promoter. (D) <t>JNK1</t> activation by LMP1 is independent of TRADD. HA-JNK1 immunocomplex kinase assays in DG75 TRADD +/+ and TRADD –/– cells are shown. Immunoprecipitated HA-JNK1 was dectected with the anti-JNK1 (C17) antibody.
Dhea S Enzyme Immunoassay Kit, supplied by Salimetrics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chitin resin new england biolabs cat  (New England Biolabs)
96
New England Biolabs chitin resin new england biolabs cat
(A) The knockout of TRADD in DG75 B lymphocytes abolishes TNFR1 activation of the NF-κB pathway. DG75 TRADD +/+ or TRADD –/– cells were stimulated with 200 ng ml −1 soluble human TNFα (Roche) for the indicated times. Levels of phospho-I-κB and I-κB were analyzed on immunoblots. αTubulin served as the loading control. (B) LMP1 activation of IKKβ requires TRADD. DG75 TRADD +/+ or TRADD –/– cells were electroporated with the indicated LMP1 constructs and Flag-IKKβ. Flag-IKKβ activity was monitored in immunocomplex kinase assays. GST-I-κBα phosphorylation was quantified by a phosphoimager and is given as x-fold induction. Immunoprecipitated Flag-IKKβ and the expressed HA-LMP1 constructs were detected using the anti-Flag (M2) and anti-HA (12CA5) antibodies, respectively. LMP1-CD40 was visualized by the anti-CD40 antibody. IB, immunoblot; IP, immunoprecipitation. (C) Ectopic TRADD expression rescues LMP1 activation of IKKβ in TRADD–/– cells. IKKβ assays were performed in DG75 TRADD–/– cells as in (B). Where indicated, TRADD was expressed at physiological levels by co-transfecting 1 μg of pACYC184-1012.4, which carries the complete human TRADD gene including the TRADD promoter. (D) <t>JNK1</t> activation by LMP1 is independent of TRADD. HA-JNK1 immunocomplex kinase assays in DG75 TRADD +/+ and TRADD –/– cells are shown. Immunoprecipitated HA-JNK1 was dectected with the anti-JNK1 (C17) antibody.
Chitin Resin New England Biolabs Cat, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) The knockout of TRADD in DG75 B lymphocytes abolishes TNFR1 activation of the NF-κB pathway. DG75 TRADD +/+ or TRADD –/– cells were stimulated with 200 ng ml −1 soluble human TNFα (Roche) for the indicated times. Levels of phospho-I-κB and I-κB were analyzed on immunoblots. αTubulin served as the loading control. (B) LMP1 activation of IKKβ requires TRADD. DG75 TRADD +/+ or TRADD –/– cells were electroporated with the indicated LMP1 constructs and Flag-IKKβ. Flag-IKKβ activity was monitored in immunocomplex kinase assays. GST-I-κBα phosphorylation was quantified by a phosphoimager and is given as x-fold induction. Immunoprecipitated Flag-IKKβ and the expressed HA-LMP1 constructs were detected using the anti-Flag (M2) and anti-HA (12CA5) antibodies, respectively. LMP1-CD40 was visualized by the anti-CD40 antibody. IB, immunoblot; IP, immunoprecipitation. (C) Ectopic TRADD expression rescues LMP1 activation of IKKβ in TRADD–/– cells. IKKβ assays were performed in DG75 TRADD–/– cells as in (B). Where indicated, TRADD was expressed at physiological levels by co-transfecting 1 μg of pACYC184-1012.4, which carries the complete human TRADD gene including the TRADD promoter. (D) JNK1 activation by LMP1 is independent of TRADD. HA-JNK1 immunocomplex kinase assays in DG75 TRADD +/+ and TRADD –/– cells are shown. Immunoprecipitated HA-JNK1 was dectected with the anti-JNK1 (C17) antibody.

Journal: PLoS Biology

Article Title: The Viral Oncoprotein LMP1 Exploits TRADD for Signaling by Masking Its Apoptotic Activity

doi: 10.1371/journal.pbio.0060008

Figure Lengend Snippet: (A) The knockout of TRADD in DG75 B lymphocytes abolishes TNFR1 activation of the NF-κB pathway. DG75 TRADD +/+ or TRADD –/– cells were stimulated with 200 ng ml −1 soluble human TNFα (Roche) for the indicated times. Levels of phospho-I-κB and I-κB were analyzed on immunoblots. αTubulin served as the loading control. (B) LMP1 activation of IKKβ requires TRADD. DG75 TRADD +/+ or TRADD –/– cells were electroporated with the indicated LMP1 constructs and Flag-IKKβ. Flag-IKKβ activity was monitored in immunocomplex kinase assays. GST-I-κBα phosphorylation was quantified by a phosphoimager and is given as x-fold induction. Immunoprecipitated Flag-IKKβ and the expressed HA-LMP1 constructs were detected using the anti-Flag (M2) and anti-HA (12CA5) antibodies, respectively. LMP1-CD40 was visualized by the anti-CD40 antibody. IB, immunoblot; IP, immunoprecipitation. (C) Ectopic TRADD expression rescues LMP1 activation of IKKβ in TRADD–/– cells. IKKβ assays were performed in DG75 TRADD–/– cells as in (B). Where indicated, TRADD was expressed at physiological levels by co-transfecting 1 μg of pACYC184-1012.4, which carries the complete human TRADD gene including the TRADD promoter. (D) JNK1 activation by LMP1 is independent of TRADD. HA-JNK1 immunocomplex kinase assays in DG75 TRADD +/+ and TRADD –/– cells are shown. Immunoprecipitated HA-JNK1 was dectected with the anti-JNK1 (C17) antibody.

Article Snippet: The following primary antibodies were used: Actin (I-19), CD40 (C-20), I-κBα (C-21), IKKβ (H-470), JNK1 (C-17), TNFR1 (H-5), TRADD (H-278), αTubulin (B-5-1-2) (all from Santa Cruz Biotech.), HA (12CA5) (Roche), Flag (M2) (Sigma), TRADD (mouse, BD Transduction Lab.), and phospho-I-κBα(Ser32) (New England Biolabs).

Techniques: Knock-Out, Activation Assay, Western Blot, Control, Construct, Activity Assay, Phospho-proteomics, Immunoprecipitation, Expressing